Expanded Bed Chromatography - Livro de bolso

EAN (ISBN-13): 9780792361237
ISBN (ISBN-10): 0792361237
Livro de capa dura
Livro de bolso
Ano de publicação: 1999
Editor/Editora: Springer Netherlands
280 Páginas
Peso: 0,725 kg
Língua: eng/Englisch

Livro na base de dados desde 2008-04-01T22:51:56+01:00 (Lisbon)
Página de detalhes modificada pela última vez em 2024-01-22T06:08:06+00:00 (Lisbon)
Número ISBN/EAN: 0792361237

Número ISBN - Ortografia alternativa:
0-7923-6123-7, 978-0-7923-6123-7
Ortografia alternativa e termos de pesquisa relacionados:
Autor do livro: temam
Título do livro: expanded bed chromatography, chroma, going bed, bed with, and bed, the science mechanics, focusing, for data science, computer, artificial intelligence

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Dados da editora

Autor: B. Mattiasson
Título: Expanded Bed Chromatography
Editora: Springer; Springer Netherland
272 Páginas
Ano de publicação: 1999-12-31
Dordrecht; NL
Língua: Inglês
106,99 € (DE)
109,99 € (AT)
118,00 CHF (CH)
Available
VI, 272 p.

BB; Hardcover, Softcover / Technik/Chemische Technik; Biotechnologie; Verstehen; Chromatography; DNA; Downstream Processing; Fermentation; Glucose; Scale up; gene therapy; proteins; biochemical engineering; Chemical Bioengineering; Physical Chemistry; Biotechnology; Physikalische Chemie; BC; EA

Expanded bed adsorption: elution in expanded bed mode.- Monitoring, modeling, and control strategies for expanded-bed adsorption processes.- Factors affecting dispersion in expanded bed chromatography.- Physical and biochemical characterization of a simple intermediate between fluidized and expanded bed contactors.- On-line monitoring of the purification of GST-(His)6 from an unclarified Escherichia coli homogenate within an immobilised metal affinity expanded bed.- The effect of column verticality on separation efficiency in expanded bed adsorption.- Evaluation of the effect of in-bed sampling on expanded bed adsorption.- EBA columns with a distribution system based on local stirring.- Simplified and more robust EBA processes by elution in expanded bed mode.- Cell/adsorbent interactions in expanded bed adsorption of proteins.- Validation issues related to expanded bed technology.- Highly dense beaded sorbents suitable for fluidized bed applications.- Characterization of very dense mineral oxide—gel composites for fluidized-bed adsorption of biomolecules.- Expanded bed adsorption on supermacroporous cross-linked cellulose matrix.- Characterisation of STREAMLINETM Phenyl.- Use of Streamline chelating for capture and purification of poly-His-tagged recombinant proteins.- A simplified purification procedure of a-lactalbumin from milk using Ca2+-dependent adsorption in hydrophobic expanded bed chromatography.- Expanded bed adsorption as a unique unit operation for the isolation of bacteriocins from fermentation media.- Pilot scale recovery of monoclonal antibodies by expanded bed ion exchange adsorption.- Capture of human Fab fragments by expanded bed adsorption with a mixed mode adsorbent.- Purification of Recombinant Human Serum Albumin. Efficient purification usingSTREAMLINE.- Direct coupling of expanded bed adsorption with a downstream purification step.- Production of plasmid DNA for human gene therapy using modified alkaline cell lysis and expanded bed anion exchange chromatography.- Development and scale up of a capture step (expanded bed chromatography) for a fusion protein expressed intracellularly in Escherichia coli.- On-line monitoring of glucose and/or lactate in a fermentation process using an expanded micro-bed flow injection analyzer.- Binding assays in heterogeneous media using a flow injection system with an expanded micro-bed adsorption column.- Use of a micro-expanded bed containing immobilised lysozyme for cell disruption in flow injection analysis.- Flow injection analysis of intracellular ?-galactosidase in Escherichia coli cultivations, using an on-line system including cell disruption, debris separation and immunochemical quantification.- Key word Index.- Author Index.