ISBN: 9780896038080

The yeast two-hybrid system is one of the most widely used and productive techniques available for investigating the macromolecular interactions that affect virtually all biological proce… mais…

The yeast two-hybrid system is one of the most widely used and productive techniques available for investigating the macromolecular interactions that affect virtually all biological processes. In Two-Hybrid Systems: Methods and Protocols, Paul N. MacDonald has assembled a collection of these powerful molecular tools for examining and characterizing protein-protein, protein-DNA, and protein-RNA interactions. The techniques range from the most basic (introducing plasmids into yeasts, interaction assays, and recovering the plasmids from yeast) to the most advanced alternative strategies (involving one-hybrid, split two-hybrid, three-hybrid, membrane recruitment systems, and mammalian systems). Methods are also provided for dealing with the well-known problems of artifacts and false positives and for identifying the interacting partners in important biological systems, including the Smad and nuclear receptor pathways. To ensure ready reproducibility and robust results, each technique is described in step-by-step detail by researchers who employ it regularly. Comprehensive and highly practical, Two-Hybrid Systems: Methods and Protocols not only reveals how the great variety of plasmid vectors and approaches may be optimally deployed, but also quickly empowers novices to establish two-hybrid systems in their laboratories, and experienced researchers to expand their repertoire of techniques., Humana<

2001, ISBN: 9780896038080

Editor: MacDonald, Paul N. Humana, Paperback, Auflage: 2001, 348 Seiten, Publiziert: 2001-05-17T00:00:01Z, Produktgruppe: Book, 1.14 kg, Biochemistry, Biological Sciences, Science & Math,… mais…

Editor: MacDonald, Paul N. Humana, Paperback, Auflage: 2001, 348 Seiten, Publiziert: 2001-05-17T00:00:01Z, Produktgruppe: Book, 1.14 kg, Biochemistry, Biological Sciences, Science & Math, Subjects, Books, Cell Biology, Microbiology, Molecular Biology, Research, Medical Books, General, Medicine & Health Sciences, New, Used & Rental Textbooks, Specialty Boutique, Biology & Life Sciences, Science & Mathematics, Format: Illustrated, Humana, 2001<

2001, ISBN: 9780896038080

Trade paperback, New Book Original US edition, Perfect Condition. Printed in English. Excellent Quality, Service and customer satisfaction guaranteed., Brand New, [PU: Humana]

ISBN: 9780896038080

Humana Press . Papeback. New. pp. 352, Humana Press, 6