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Erscheinungsdatum: 09.08.2012, Medium: Taschenbuch, Einband: Kartoniert / Broschiert, Titel: Plant MicroRNAs, Titelzusatz: Methods and Protocols, Auflage: 2010, Redaktion: Green, Pamela J… mais…
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2012, ISBN: 9781617796531
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2012, ISBN: 9781617796531
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2012, ISBN: 9781617796531
MicroRNAs constitute a particularly important class of small RNAs given their abundance, broad phylogenetic conservation and strong regulatory effects, with plant miRNAs uniquely divulgin… mais…
ISBN: 9781617796531
MicroRNAs constitute a particularly important class of small RNAs given their abundance, broad phylogenetic conservation and strong regulatory effects, with plant miRNAs uniquely divulgin… mais…
2012
ISBN: 9781617796531
Erscheinungsdatum: 09.08.2012, Medium: Taschenbuch, Einband: Kartoniert / Broschiert, Titel: Plant MicroRNAs, Titelzusatz: Methods and Protocols, Auflage: 2010, Redaktion: Green, Pamela J… mais…
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2012, ISBN: 9781617796531
Humana Press, 2012-08-09. 2010. Paperback. Good., Humana Press, 2012-08-09, 2.5
2012, ISBN: 9781617796531
Methods and Protocols, Buch, Softcover, 2010 ed. [PU: Humana Press Inc.], Humana Press Inc., 2012
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MicroRNAs constitute a particularly important class of small RNAs given their abundance, broad phylogenetic conservation and strong regulatory effects, with plant miRNAs uniquely divulging their ancient evolutionary origins and their strong post-transcriptional regulatory effects. In Plant MicroRNAs: Methods and Protocols, experts in the field present chapters that focus on the identification, validation, and characterization of the miRNA class of RNAs, and address important aspects about heterochromatic small interfering RNAs. In addition, the methods contained in this volume emphasize miRNA analyses, but also include ways to distinguish one class of small RNAs from another. As a volume in the highly successful Methods in Molecular Biology™ series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easy to use, Plant MicroRNAs: Methods and Protocols provides the research community with a set of protocols that will help advance vital miRNA research for all plant species, both in typical model species and non-model species alike.
Dados detalhados do livro - Plant MicroRNAs
EAN (ISBN-13): 9781617796531
ISBN (ISBN-10): 1617796530
Livro de capa dura
Livro de bolso
Ano de publicação: 2012
Editor/Editora: Humana Press Inc.
280 Páginas
Peso: 0,530 kg
Língua: Englisch
Livro na base de dados desde 2008-11-23T07:15:13+00:00 (Lisbon)
Página de detalhes modificada pela última vez em 2023-11-16T16:34:48+00:00 (Lisbon)
Número ISBN/EAN: 1617796530
Número ISBN - Ortografia alternativa:
1-61779-653-0, 978-1-61779-653-1
Ortografia alternativa e termos de pesquisa relacionados:
Autor do livro: von meyers, blake, meyer, green
Título do livro: plant biology, molecular
Dados da editora
Autor: Blake C. Meyers; Pamela J. Green
Título: Methods in Molecular Biology; Plant MicroRNAs - Methods and Protocols
Editora: Humana; Humana Press
261 Páginas
Ano de publicação: 2012-08-09
Totowa; NJ; US
Impresso / Feito em
Língua: Inglês
128,39 € (DE)
131,99 € (AT)
142,00 CHF (CH)
POD
X, 261 p. 46 illus.
BC; Hardcover, Softcover / Biologie/Biochemie, Biophysik; Biochemie; Verstehen; Arabidopsis; Evolution; Heterochromatic siRNAs; Post-transcriptional regulatory effects; Promoter; RNA purification; Regulation; Transcriptome; arabidopsis thaliana; bioinformatics; conservation; genes; miRNAs; molecular biology; transcription; Biochemistry; Plant Science; Plant Genetics; Medical Genetics; Botanik und Pflanzenwissenschaften; Pflanzenbiologie; Genetik (nicht-medizinisch); Genetik, Medizin; BB; EA
Piecing the Puzzle Together: Genetic Requirements for miRNA Biogenesis in Arabidopsis thaliana.- Prediction of Plant miRNA Genes.- Methods for Isolation of Total RNA to Recover miRNAs and Other Small RNAs from Diverse Species.- miRNA Target Prediction in Plants.- A Method to Discover Phased siRNA Loci.- Directed Gene Silencing with Artificial MicroRNAs.- Bioinformatics Analysis of Small RNAs in Plants Using Next Generation Sequencing Technologies.- High-Throughput Approaches for miRNA Expression Analysis.- In Situ Detection of miRNAs Using LNA Probes.- Analysis of miRNA Modifications.- MicroRNA Promoter Analysis.- Computational Methods for Comparative Analysis of Plant Small RNAs.- Biotic Stress-Associated microRNAs: Identification, Detection, Regulation, and Functional Analysis.- Abiotic Stress-Associated miRNAs: Detection and Functional Analysis.- Processing of miRNA Precursors.- Purification of Arabidopsis Argonaute Complexes and Associated Small RNAs.- Transient Assays for the Analysis of miRNA Processing and Function.Presents fundamentals of plant miRNA biology, including pathways and approaches used for their discovery and investigation, as written by leading researchers in the field Covers a broad range of molecular approaches to the study of small RNA, from basic to advanced experimental and computational techniques Details the methods by which the expression of miRNAs and their impact on mRNA levels is characterized, including deep sequencing methods for miRNAs and target RNAs Enables a molecular biologist to design and perform experiments on the biology of plant miRNAs and small RNAs, including detection, quantification and localization, as well as approaches to data analysis Includes applications and advanced adaptations of techniques relevant to all plant biologists Includes supplementary material: sn.pub/extras
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